Fig 1: Effect of miR-148b-3p on the endogenous protein expression of ZNF804A in SH-SY5Y cells. (A) Western blotting results demonstrated the effects of the miR-148b-3p mimic treatment on ZNF804A expression. (B) Western blotting revealed the effects of the miR-148b-3p inhibitor treatment on ZNF804A expression. (C) Quantification of western blotting results presented in A. (D) Quantification of western blotting results presented in B. GAPDH was used as an internal control in both experiments. Statistical analyses were performed on three independent results. *P<0.05, **P<0.01. miR, microRNA; ZNF804A, zinc finger protein 804A; NC, negative control.
Fig 2: Venn diagrams of bioinformatics results for miR-148b-3p and ZNF804A. (A) ZNF804A is one of 23 shared target genes among the three databases. (B) miR-148b-3p is one of three shared miRNAs among the three databases. miR/miRNA, microRNA; ZNF804A, zinc finger protein 804A.
Fig 3: miR-148b-3p regulation of COMT and PRSS16 is rescued by ZNF804A in SH-SY5Y cells. (A) Infection results of recombinant ZNF804A adenovirus. Multiplicity of infection=50. (B) mRNA expression of ZNF804A in SH-SY5Y cells. (C) mRNA expression of COMT in SH-SY5Y cells. (D) mRNA expression of PRSS16 in SH-SY5Y cells. *P<0.05, **P<0.01, ***P<0.001. miR, microRNA; ZNF804A, zinc finger protein 804A; NC, negative control; COMT, catechol-O-methyltransferase; PRSS16, serine protease 16.
Fig 4: Effect of miR-148b-3p on the expression levels of COMT and PRSS16 in SH-SY5Y cells. (A) mRNA expression of COMT in SH-SY5Y cells. (B) mRNA expression of PRSS16 in SH-SY5Y cells. *P<0.05, ****P<0.0001. miR, microRNA; ZNF804A, zinc finger protein 804A; NC, negative control; COMT, catechol-O-methyltransferase; PRSS16, serine protease 16.
Fig 5: Altered expression levels of miR-148b-3p and ZNF804A in the peripheral blood of patients with schizophrenia and healthy controls. (A) Increased expression of miR-148b-3p. (B) Decreased expression of ZNF804A. (C) Correlation analysis between miR-148b-3p and ZNF804A. *P<0.05, **P<0.01. miR/miRNA, microRNA; ZNF804A, zinc finger protein 804A.
Supplier Page from Abcam for Anti-ZNF804A antibody